Coding
Part:BBa_K4220008:Design
Designed by: Xiaoya Liu Group: iGEM22_NEU_CHINA (2022-09-25)
P2Y2-P2A-mCherry
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1159
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1095
Illegal AgeI site found at 389 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 570
Illegal SapI site found at 615
Design Notes
In order to improve the capability for P2Y2 to be responsiveness to extracellular ATP (eATP), substitutions (K240N and F307S) will be applied.
Source
P2Y2: human mCherry: mammalian